HPLC is a technique for separation, identification and quantification of components in a mixture. It is especially suitable for compounds which are not easily volatilized, thermally unstable and have high molecular weights.
The liquid phase is pumped at a constant rate to the column packed with the stationary phase. Before entering the column the analysis sample is injected into the carrier stream. On reaching the column the sample components are selectively retained on the basis of physic-chemical interactions between the analytic molecules and the stationary phase. The mobile phase moving at a steady rate elutes the components based on the operating conditions. Detection techniques are employed for detection and quantification of the eluted components.
High performance liquid chromatography is basically a highly improved form of column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster.
It also allows you to use a very much smaller particle size for the column packing material which gives a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This allows a much better separation of the components of the mixture.
The other major improvement over column chromatography concerns the detection methods which can be used. These methods are highly automated and extremely sensitive.
Upon successful completion of this course, the delegates will be able to:
This course is intended for Laboratory personnel and technical staff such as chemists, Analysts, chemical engineers, and anybody interested in chemical analysis, research and development, environmental studies, quality control, refineries, petrochemical plants, water and wastewater plants, hospitals and medical centers.
Day 1 Introduction
Practical and Theoretical Principles
Performing an Analysis
Day 2
Separation Fundamentals and Mobile Phase
Day 3 Gradient Elution
HPLC Instrumentation
Detectors ankd Qualitative and Quantitative Analysis Day 4
Day 5 Separation Modes and Columns
Quantitative Analysis and Troubleshooting
Gradients and Method Development
Using this course overview, the instructor(s) will brief participants about the course topics that were covered during the course.
International Center for Training & Development (ICTD) will award an internationally recognized certificate(s) for each delegate on completion of training.
A variety of methodologies will be used during the course that includes:
This rate includes participant’s manual, Hand-Outs, buffet lunch, coffee/tea on arrival, morning & afternoon of each day.
Daily Course Timings
08:00 - 08:20 Morning Coffee / Tea
08:20 - 10:00 First Session
10:00 - 10:20 Coffee / Tea / Snacks
10:20 - 12:20 Second Session
12:20 - 13:30 Lunch Break & Prayer Break
13:30 - 15:00 Last Session
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