Gas and Liquid Chromatography and Troubleshooting

This is a comprehensive course covering the following areas:

• Sample preparation
• Sample introduction
• Sample separation
• Columns
• Detectors
• Data acquisition
• Troubleshooting
• Reporting

Objectives

• Understanding the basic theoretical aspects of Gas Chromatography.
• Communicate practical information, capabilities, and limitations of Gas & Liquid Chromatography
• Acquire confidence in the analysis technique, troubleshooting, and analytical results evaluation.

Course Outline

Day 1

Introduction to Chromatography

• The History of Chromatography – GC & LC
• Overview of GC & LC Chromatography.
• The Modern Chromatograph.
• Chromatography Nomenclature.
• Factors Controlling Retention.
• Effects of Stationary Phase Loading on the Performance of a Chromatographic System.
• Liquid Chromatography – The Development Process.
• Molecular Forces and Chromatographic Selectivity.

Day 2

Chromatography Basics

• Basic Chromatography.
• Sample Introduction.
• The role of sample introduction and injection ports in GC operations.
• Injection ports maintenance and its impact on GC performance.
• Columns.
• The role of columns in GC operations.
• Column selection & maintenance.
• How columns can impact GC performance.

Day 3

GC Operation

• Peak Dispersion in a Chromatographic Column.
• Detector Selection.
• The role of Detectors in GC operations.
• Detector maintenance.
• How detectors can impact GC performance.
• Setup and GC. Operation, Basic steps.
• Preparation for operation.
• Sampling Techniques.
• Data Acquisition and Calibration.

Day 4

Calibration

• Data Acquisition and Processing System.
• Calibration linked to GC performance.
• Applications and troubleshooting.

Day 5

Chromatography Applications

• Method Development.
• Gas Chromatography.
• Liquid Chromatography.
• ISO17025 Accreditation Basics.
• Laboratory Management & Troubleshooting.